376 papers
This study utilized DArTSeq genotyping and QTL mapping in a kenaf F2 population to identify significant and putative genetic loci controlling oil sorption capacity, thereby providing a foundation for marker-assisted selection to develop improved genotypes for oil spill remediation.
This study demonstrates that ATG5 drives steroid-induced osteonecrosis of the femoral head by triggering excessive autophagy and PERK-mediated endoplasmic reticulum stress leading to apoptosis, and shows that targeted ATG5 silencing effectively blocks this pathological cascade to alleviate bone damage.
This study reveals that the ATAD2 bromodomain mediates liquid-liquid phase separation to promote histone H4 acetylation, thereby facilitating chromatin remodeling and upregulating key genes such as C-MYC, CCND3, and ATF2.
This study reports the discovery and optimization of bicyclic peptides that effectively neutralize *Staphylococcus aureus* α-hemolysin by blocking its binding to host cells, offering a promising, synthetically accessible anti-virulence therapeutic strategy.
This study validates shotgun metagenomics for detecting soil-transmitted helminths in faecal samples, demonstrating that mitochondrial sequence mapping combined with short-read sequencing offers the highest sensitivity and specificity, although detection remains challenging for low-intensity infections.
This study establishes that positive DNA supercoiling, generated by transcription, R-loops, and Cohesin during interphase and by Condensins during mitosis, accumulates at key regulatory elements to serve as a topological memory that links DNA mechanics to transcriptional control, genome architecture, and epigenetic inheritance.
This study elucidates the evolutionary divergence in eukaryotic DNA replication licensing by demonstrating that *Yarrowia lipolytica* utilizes a highly plastic ORC-Cdc6 complex to recognize variable origin sequences, contrasting with the rigid sequence-specific recognition in *S. cerevisiae* and providing structural insights into the conserved yet adaptable mechanisms of origin identification across species.
This study identifies the *E. coli* lipoprotein YbjP as a structural partner that binds to and stabilizes the outer membrane protein TolC, mimicking the lipid modifications found in homologs from other bacteria, although this interaction is not strictly required for standard efflux activity.
This study demonstrates that in *C. elegans*, lysosomal expansion compartments serve as a general mechanism for sequestering excess zinc, copper, and manganese to prevent metal toxicity, a process mediated by specific transporters and characterized by the remodeling of lysosomal volume.
This paper introduces a novel cryo-lamella preparation workflow that integrates crack-arrest perforations and soft-suspension ring springs to mechanically reinforce fragile specimens, thereby significantly reducing crack propagation and fracture during cryo-TEM imaging.
This study reveals that during Sendai virus infection, the human RNA helicase DDX3X maintains its binding to host GC-rich 5'UTRs while acquiring new targets like IFNB1 to directly promote interferon-beta translation, thereby defining a novel post-transcriptional mechanism in antiviral immunity without significantly impacting the viral life cycle.
This study demonstrates that m6A modification critically regulates the inverse balance between HPV16 circE7 and E6*I splicing, where circE7 production is essential for viral replication while its suppression enhances cellular transformation.
This study establishes the first MS2-MCP-based single-molecule RNA imaging system in *Schizosaccharomyces pombe* by systematically optimizing MCP expression and localization with tandem StayGold tags, thereby enabling quantitative analysis of RNA dynamics in this key eukaryotic model organism.
This study presents a cost-effective, in-house production and purification protocol for a redesigned MarathonRT enzyme that matches the performance of commercial alternatives (Induro and uMRT) in tRNA sequencing, while also introducing a rapid spin-column extraction method to further reduce the time and cost of library preparation.
This study elucidates that the circZNF827-hnRNPL/K complex transcriptionally represses NGFR and other loci enriched with H3K27me3 marks during neuronal differentiation, triggering a primary regulatory event that initiates a secondary response to further promote the differentiation process.
This study presents an optimized tRNA Structure-seq pipeline that integrates natural tRNA modifications and refined energy parameters to achieve 94% accuracy in predicting *S. cerevisiae* tRNA secondary structures, revealing that these molecules maintain their canonical cloverleaf conformation and structural robustness even under mild stress conditions.
Using a surgically induced mouse model, this study demonstrates that preconceptional immunomodulation partially reverses endometriosis-induced pregnancy complications and normalizes specific transcriptional alterations in the fetal-maternal interface, suggesting that targeting the immune system could improve pregnancy outcomes for women with endometriosis.
This speculative review synthesizes the discovery and multifunctional roles of Igf2bp proteins in nervous system development and cancer, proposing a model where these RNA-binding proteins evolved to coordinate post-transcriptional regulation in neurogenesis and were later co-opted in tumorigenesis, a hypothesis supported by evolutionary analysis revealing that genes co-evolved with Igf2bps are enriched for neural functions and overlap with oncogenic targets stabilized by Igf2bp1.
The authors developed a one-pot, isothermal MethylAmp workflow that simultaneously amplifies DNA and preserves native methylation patterns by optimizing a unified 42°C reaction condition to support both helicase-dependent amplification and DNMT1-mediated methylation, thereby enabling accurate epigenetic analysis without disrupting methylation marks.
This study utilizes CRISPR screening to map the genetic architecture of homologous recombination sub-pathways following PARP inhibitor treatment, revealing distinct roles for factors like RAD54L and RAD51AP1 and demonstrating that TOP3A deficiency drives a switch from synthesis-dependent strand annealing to the double Holliday junction pathway by abolishing the requirement for ATRX and H3.3.